Plasmid

Part:BBa_K3776021

Designed by: Helene Heling Huang   Group: iGEM21_HK_SSC   (2021-10-01)


panS-SPIN-CF3BD

This part allows genome insertion of a cyclase CF3BD ( BBa_K3776012) that catalyzes the cyclization of 5-aminovalerate(5AVA) to valerolactam in Synechococcus elongatus UTEX 2973. The INTEGRATE system ( BBa_K3776019) that insert three genes as a donor DNA into the genome of S.elongatus UTEX 2973 is cloned into the panS-loxP-MCS shuttle vector ( BBa_K3776009). The shuttle vector can be replicated in E.coli for plasmid miniprep and in S.elongatus UTEX 2973 for expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7310
    Illegal NheI site found at 9397
    Illegal NheI site found at 13515
    Illegal NotI site found at 32
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 205
    Illegal BglII site found at 6736
    Illegal BglII site found at 7527
    Illegal BglII site found at 8057
    Illegal BglII site found at 13302
    Illegal BamHI site found at 6
    Illegal BamHI site found at 5879
    Illegal BamHI site found at 14430
    Illegal BamHI site found at 15262
    Illegal XhoI site found at 5662
    Illegal XhoI site found at 14413
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2377
    Illegal AgeI site found at 4102
    Illegal AgeI site found at 13434
    Illegal AgeI site found at 15768
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5845
    Illegal BsaI.rc site found at 5819
    Illegal SapI.rc site found at 8813


[edit]
Categories
Parameters
None